Patient-derived > Asan Preclinical Evaluation center for cancer therapeutiX

List of Models

Patient-Derived primary cells (PPC)

Panels : liver cancer, ovarian cancer, colorectal cancer, gastric cancer, prostate cancer, lung cancer
- B-raf, EGFR, Her2, K-ras, PI3K, Diagnostic assays for HRD, Androgen receptor etc.

Clinically annotated patient-derived model

Patient-derived models with patient information and whole exome sequencing

Patient-derived xenografts(PDX)

Panels : colorectal cancer, liver cancer, lung cancer, pancreatic cancer, gastric cancer, renal cell carcinoma, ovarian cancer, uterine cancer, bile duct cancer, prostate cancer etc.
- Lung Cancer: Genomic analysis (e.g., whole exome sequencing), radiation therapy response data provided
- Gastric Cancer: HER2-positive & Herceptin response data, genomic analysis results provided
- Colorectal Cancer: Radiation therapy response data, Irinotecan response data provided
- Pancreatic Cancer: Gemcitabine response data provided
- Ovarian Cancer: Olaparib response data, genomic analysis results provided
- Liver Cancer: Sorafenib response data provided
- Cholangiocarcinoma: FGFR4-positive, etc.
- Kidney Cancer: Sunitinib response data provided

Patient-derived organoid (PDO)

Panels: Colorectal Cancer, Liver Cancer, Lung Cancer, Gastric Cancer, and normal colon/liver/lung/gastric models.

CPatient-derived models with available clinical data and mutation profiling, including K-ras, EGFR, Her2 mutations, and Homologous Recombination Deficiency (HRD) diagnostics.

Patient-derived models with clinical data and results from whole exome sequencing and RNA sequencing.
- Models with data on PD-L1 expression and gene expression pattern analysis

Patient specimens

Clinically annotated frozen tumor tissues, normal tissues, and blood samples

Tumor and normal tissues in paraffin-embedded and frozen formats

Service

  • Customized model selection and study design
  • Biomarker validation
  • Mechanism of action (MOA) and proof of concept (POC) studies
  • Efficacy evaluation based on genetic characteristics
  • In vitro drug efficacy testing
  • Cell viability, cell death assays, and synergy index analysis
  • Signal pathway analysis: Immunocytochemistry (ICC), Western blotting, immunoprecipitation, ELISA, quantitative PCR (qPCR)
  • Patient-derived xenograft (PDX) models using organoids:
    Simultaneous efficacy evaluationin vitro and in vivo models
  • Tumor growth inhibition (TGI) and tumor growth delay (TGD) studies
  • Kaplan-Meier survival analysis
  • Radiotherapy response evaluation
  • Histological analysis
  • Tissue preparation, immunohistochemistry (IHC), TUNEL assay, and DNA/RNA/protein extraction

Service example

Utilizing patient-derived models tailored to drug characteristics

Evaluation of tumor growth inhibition efficacy by drug treatment